Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1130916..1131643 Replicon chromosome
Accession NZ_CP116931
Organism Escherichia coli strain MLI106K1

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag NL422_RS06065 Protein ID WP_000547555.1
Coordinates 1130916..1131227 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL422_RS06070 Protein ID WP_000126294.1
Coordinates 1131224..1131643 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL422_RS06040 (1126851) 1126851..1128560 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
NL422_RS06045 (1128570) 1128570..1129112 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
NL422_RS06050 (1129112) 1129112..1129879 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
NL422_RS06055 (1129876) 1129876..1130286 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
NL422_RS06060 (1130279) 1130279..1130749 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
NL422_RS06065 (1130916) 1130916..1131227 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
NL422_RS06070 (1131224) 1131224..1131643 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
NL422_RS06075 (1131722) 1131722..1133146 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
NL422_RS06080 (1133155) 1133155..1134612 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
NL422_RS06085 (1134872) 1134872..1135882 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
NL422_RS06090 (1136031) 1136031..1136558 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T269496 WP_000547555.1 NZ_CP116931:1130916-1131227 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT269496 WP_000126294.1 NZ_CP116931:1131224-1131643 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References