Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3528607..3529301 Replicon chromosome
Accession NZ_CP116929
Organism Escherichia coli strain MLI105

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag NL421_RS17575 Protein ID WP_001263489.1
Coordinates 3528607..3529005 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NL421_RS17580 Protein ID WP_000554758.1
Coordinates 3529008..3529301 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3524195) 3524195..3524275 - 81 NuclAT_13 - -
- (3524195) 3524195..3524275 - 81 NuclAT_13 - -
- (3524195) 3524195..3524275 - 81 NuclAT_13 - -
- (3524195) 3524195..3524275 - 81 NuclAT_13 - -
NL421_RS17550 (3524871) 3524871..3525329 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NL421_RS17555 (3525590) 3525590..3527047 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NL421_RS17560 (3527104) 3527104..3527625 - 522 Protein_3401 peptide chain release factor H -
NL421_RS17565 (3527621) 3527621..3527827 - 207 Protein_3402 RtcB family protein -
NL421_RS17570 (3528145) 3528145..3528597 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
NL421_RS17575 (3528607) 3528607..3529005 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NL421_RS17580 (3529008) 3529008..3529301 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NL421_RS17585 (3529353) 3529353..3530408 - 1056 WP_001226164.1 DNA polymerase IV -
NL421_RS17590 (3530479) 3530479..3531264 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NL421_RS17595 (3531236) 3531236..3532948 + 1713 Protein_3408 flagellar biosynthesis protein FlhA -
NL421_RS17600 (3533172) 3533172..3533669 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3478403..3534471 56068


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T269487 WP_001263489.1 NZ_CP116929:c3529005-3528607 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT269487 WP_000554758.1 NZ_CP116929:c3529301-3529008 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References