Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3833823..3834364 Replicon chromosome
Accession NZ_CP116925
Organism Escherichia coli strain MLI104K2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NL420_RS19705 Protein ID WP_000615983.1
Coordinates 3834086..3834364 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NL420_RS19700 Protein ID WP_000729703.1
Coordinates 3833823..3834083 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL420_RS19680 (3829498) 3829498..3830283 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
NL420_RS19685 (3830255) 3830255..3831967 + 1713 Protein_3753 flagellar biosynthesis protein FlhA -
NL420_RS19690 (3832191) 3832191..3832688 - 498 WP_072667535.1 REP-associated tyrosine transposase RayT -
NL420_RS19695 (3832864) 3832864..3833613 - 750 WP_024192679.1 C40 family peptidase -
NL420_RS19700 (3833823) 3833823..3834083 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NL420_RS19705 (3834086) 3834086..3834364 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NL420_RS19710 (3834520) 3834520..3835260 + 741 WP_072667536.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NL420_RS19715 (3835231) 3835231..3835998 - 768 WP_000333380.1 class II glutamine amidotransferase -
NL420_RS19720 (3836204) 3836204..3836782 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T269469 WP_000615983.1 NZ_CP116925:3834086-3834364 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT269469 WP_000729703.1 NZ_CP116925:3833823-3834083 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References