Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3728150..3728691 Replicon chromosome
Accession NZ_CP116923
Organism Escherichia coli strain MLI104K1

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NL419_RS19085 Protein ID WP_000615983.1
Coordinates 3728413..3728691 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NL419_RS19080 Protein ID WP_000729703.1
Coordinates 3728150..3728410 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL419_RS19060 (3723825) 3723825..3724610 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
NL419_RS19065 (3724582) 3724582..3726294 + 1713 Protein_3640 flagellar biosynthesis protein FlhA -
NL419_RS19070 (3726518) 3726518..3727015 - 498 WP_072667535.1 REP-associated tyrosine transposase RayT -
NL419_RS19075 (3727191) 3727191..3727940 - 750 WP_024192679.1 C40 family peptidase -
NL419_RS19080 (3728150) 3728150..3728410 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NL419_RS19085 (3728413) 3728413..3728691 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NL419_RS19090 (3728847) 3728847..3729587 + 741 WP_072667536.1 peptidoglycan meso-diaminopimelic acid protein amidase -
NL419_RS19095 (3729558) 3729558..3730325 - 768 WP_000333380.1 class II glutamine amidotransferase -
NL419_RS19100 (3730531) 3730531..3731109 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T269445 WP_000615983.1 NZ_CP116923:3728413-3728691 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT269445 WP_000729703.1 NZ_CP116923:3728150-3728410 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References