Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 652587..653314 Replicon chromosome
Accession NZ_CP116916
Organism Escherichia coli strain MLI023K2

Toxin (Protein)


Gene name higB Uniprot ID S1EX98
Locus tag NL417_RS03930 Protein ID WP_000550192.1
Coordinates 652587..652901 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag NL417_RS03935 Protein ID WP_000560266.1
Coordinates 652898..653314 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL417_RS03910 (648754) 648754..649740 - 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -
NL417_RS03915 (649819) 649819..650502 - 684 WP_001183046.1 vancomycin high temperature exclusion protein -
NL417_RS03920 (650579) 650579..651082 - 504 WP_001295542.1 M48 family metallopeptidase -
NL417_RS03925 (651167) 651167..652303 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
NL417_RS03930 (652587) 652587..652901 + 315 WP_000550192.1 type II toxin-antitoxin system toxin HigB Toxin
NL417_RS03935 (652898) 652898..653314 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
NL417_RS03940 (653359) 653359..655377 - 2019 WP_187770297.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
NL417_RS03945 (655803) 655803..658154 - 2352 WP_061344133.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12063.08 Da        Isoelectric Point: 10.0409

>T269397 WP_000550192.1 NZ_CP116916:652587-652901 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT269397 WP_000560266.1 NZ_CP116916:652898-653314 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EX98

Antitoxin

Source ID Structure

References