Detailed information of TA system TA026936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3547518..3548188
• Replicon: chromosome
• Accession: NC_017524
• Organism: Mycobacterium tuberculosis CTRI-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: MTCTRI2_RS16805
• Protein ID: WP_003899954.1
• Coordinates: 3547518..3547862 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: MTCTRI2_RS16810
• Protein ID: WP_003899955.1
• Coordinates: 3547859..3548188 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTCTRI2_RS16775	3542591..3543451	+	861	WP_003416628.1	alpha/beta hydrolase	-
MTCTRI2_RS16780	3543426..3543941	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
MTCTRI2_RS16785	3544181..3544474	+	294	WP_003416635.1	hypothetical protein	-
MTCTRI2_RS16790	3544762..3546051	+	1290	WP_003906996.1	ATP-binding protein	-
MTCTRI2_RS16795	3546398..3546832	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
MTCTRI2_RS16800	3546835..3547287	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MTCTRI2_RS16805	3547518..3547862	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MTCTRI2_RS16810	3547859..3548188	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
MTCTRI2_RS16815	3548726..3549073	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
MTCTRI2_RS16820	3549070..3549690	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
MTCTRI2_RS16825	3549850..3551115	-	1266	WP_003902423.1	hypothetical protein	-
MTCTRI2_RS16830	3551283..3551492	+	210	WP_003416778.1	hypothetical protein	-
MTCTRI2_RS16840	3551739..3552773	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T26936 WP_003899954.1 NC_017524:3547518-3547862 [Mycobacterium tuberculosis CTRI-2]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T26936 NC_017524:3547518-3547862 [Mycobacterium tuberculosis CTRI-2]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT26936 WP_003899955.1 NC_017524:3547859-3548188 [Mycobacterium tuberculosis CTRI-2]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT26936 NC_017524:3547859-3548188 [Mycobacterium tuberculosis CTRI-2]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6