Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2512612..2513339 Replicon chromosome
Accession NZ_CP116900
Organism Escherichia coli strain XH2172

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag PH219_RS12105 Protein ID WP_000547564.1
Coordinates 2512612..2512923 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PH219_RS12110 Protein ID WP_000126294.1
Coordinates 2512920..2513339 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PH219_RS12075 (2507754) 2507754..2509463 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
PH219_RS12080 (2509473) 2509473..2510015 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
PH219_RS12085 (2510015) 2510015..2510782 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
PH219_RS12090 (2510779) 2510779..2511189 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
PH219_RS12095 (2511182) 2511182..2511652 + 471 WP_060621146.1 hydrogenase maturation peptidase HycI -
PH219_RS12100 (2511677) 2511677..2512438 + 762 WP_001026446.1 hypothetical protein -
PH219_RS12105 (2512612) 2512612..2512923 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
PH219_RS12110 (2512920) 2512920..2513339 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
PH219_RS12115 (2513453) 2513453..2514877 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
PH219_RS12120 (2514886) 2514886..2516343 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
PH219_RS12125 (2516603) 2516603..2517613 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
PH219_RS12130 (2517762) 2517762..2518289 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T269319 WP_000547564.1 NZ_CP116900:2512612-2512923 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT269319 WP_000126294.1 NZ_CP116900:2512920-2513339 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References