Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2195282..2196150 Replicon chromosome
Accession NC_017524
Organism Mycobacterium tuberculosis CTRI-2

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag MTCTRI2_RS10225 Protein ID WP_010886136.1
Coordinates 2195282..2195659 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag MTCTRI2_RS10230 Protein ID WP_003409886.1
Coordinates 2195701..2196150 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTCTRI2_RS10175 2191071..2191523 - 453 WP_003899095.1 lipoprotein -
MTCTRI2_RS10180 2191587..2191988 + 402 WP_003409869.1 hypothetical protein -
MTCTRI2_RS10185 2191981..2192163 - 183 WP_003409870.1 hypothetical protein -
MTCTRI2_RS10190 2192277..2192627 - 351 WP_003899096.1 hypothetical protein -
MTCTRI2_RS10195 2192638..2193540 - 903 WP_003899097.1 hypothetical protein -
MTCTRI2_RS10200 2193561..2193752 - 192 WP_003409876.1 hypothetical protein -
MTCTRI2_RS10205 2193753..2194049 - 297 WP_003409877.1 hypothetical protein -
MTCTRI2_RS10210 2194289..2194504 + 216 WP_003409878.1 antitoxin -
MTCTRI2_RS10215 2194501..2194812 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
MTCTRI2_RS22475 2194786..2195307 - 522 WP_003904745.1 hypothetical protein -
MTCTRI2_RS10225 2195282..2195659 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
MTCTRI2_RS10230 2195701..2196150 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MTCTRI2_RS10235 2196147..2196692 + 546 WP_003409891.1 SecB-like chaperone -
MTCTRI2_RS10240 2196581..2197195 - 615 WP_003901296.1 hypothetical protein -
MTCTRI2_RS10245 2197244..2197540 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
MTCTRI2_RS10250 2197537..2197788 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
MTCTRI2_RS10255 2197775..2198269 + 495 WP_003899099.1 hypothetical protein -
MTCTRI2_RS10260 2198429..2198836 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
MTCTRI2_RS10265 2198840..2199112 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MTCTRI2_RS10270 2199145..2200365 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T26912 WP_010886136.1 NC_017524:2195282-2195659 [Mycobacterium tuberculosis CTRI-2]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T26912 NC_017524:2195282-2195659 [Mycobacterium tuberculosis CTRI-2]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT26912 WP_003409886.1 NC_017524:2195701-2196150 [Mycobacterium tuberculosis CTRI-2]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT26912 NC_017524:2195701-2196150 [Mycobacterium tuberculosis CTRI-2]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References