Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4393785..4394464 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name higB Uniprot ID U9XN95
Locus tag E5Q65_RS21210 Protein ID WP_000057540.1
Coordinates 4393785..4394087 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag E5Q65_RS21215 Protein ID WP_000806442.1
Coordinates 4394123..4394464 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS21190 (4389048) 4389048..4390268 - 1221 WP_001251608.1 fosmidomycin MFS transporter -
E5Q65_RS21195 (4390486) 4390486..4392138 + 1653 WP_000771768.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
E5Q65_RS21200 (4392175) 4392175..4392654 - 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
E5Q65_RS21205 (4392858) 4392858..4393652 - 795 WP_000365180.1 TraB/GumN family protein -
E5Q65_RS21210 (4393785) 4393785..4394087 + 303 WP_000057540.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E5Q65_RS21215 (4394123) 4394123..4394464 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
E5Q65_RS21220 (4394522) 4394522..4397026 - 2505 WP_023155691.1 copper-exporting P-type ATPase CopA -
E5Q65_RS21225 (4397288) 4397288..4398220 + 933 WP_023155692.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11781.35 Da        Isoelectric Point: 10.2638

>T268760 WP_000057540.1 NZ_CP116480:4393785-4394087 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT268760 WP_000806442.1 NZ_CP116480:4394123-4394464 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XN95

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References