Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4116762..4117303 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag E5Q65_RS19845 Protein ID WP_000615976.1
Coordinates 4116762..4117040 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag E5Q65_RS19850 Protein ID WP_000729704.1
Coordinates 4117043..4117303 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS19830 (4114344) 4114344..4114922 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
E5Q65_RS19835 (4115128) 4115128..4115895 + 768 WP_000333380.1 class II glutamine amidotransferase -
E5Q65_RS19840 (4115866) 4115866..4116606 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
E5Q65_RS19845 (4116762) 4116762..4117040 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
E5Q65_RS19850 (4117043) 4117043..4117303 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
E5Q65_RS19855 (4117489) 4117489..4118262 + 774 WP_000543899.1 C40 family peptidase -
E5Q65_RS19860 (4118319) 4118319..4118675 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
E5Q65_RS19865 (4118668) 4118668..4118946 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
E5Q65_RS19870 (4119051) 4119051..4121144 - 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
E5Q65_RS19875 (4121128) 4121128..4122267 - 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T268758 WP_000615976.1 NZ_CP116480:c4117040-4116762 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT268758 WP_000729704.1 NZ_CP116480:c4117303-4117043 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References