Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3257673..3258275 Replicon chromosome
Accession NZ_CP116480
Organism Escherichia coli strain GTEN_24

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag E5Q65_RS15775 Protein ID WP_000897305.1
Coordinates 3257673..3257984 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag E5Q65_RS15780 Protein ID WP_000356397.1
Coordinates 3257985..3258275 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Q65_RS15745 (3252703) 3252703..3253488 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
E5Q65_RS15750 (3253587) 3253587..3254186 + 600 WP_001295269.1 glucose-1-phosphatase -
E5Q65_RS15755 (3254180) 3254180..3255052 + 873 WP_000920762.1 virulence factor BrkB family protein -
E5Q65_RS15760 (3255049) 3255049..3255486 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
E5Q65_RS15765 (3255531) 3255531..3256472 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
E5Q65_RS15770 (3256536) 3256536..3257444 - 909 WP_001162704.1 alpha/beta hydrolase -
E5Q65_RS15775 (3257673) 3257673..3257984 + 312 WP_000897305.1 hypothetical protein Toxin
E5Q65_RS15780 (3257985) 3257985..3258275 + 291 WP_000356397.1 NadS family protein Antitoxin
E5Q65_RS15785 (3258861) 3258861..3259079 + 219 WP_001295676.1 CopG family transcriptional regulator -
E5Q65_RS15790 (3259298) 3259298..3259540 + 243 WP_001086388.1 protein YiiF -
E5Q65_RS15795 (3259870) 3259870..3260799 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
E5Q65_RS15800 (3260796) 3260796..3261431 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
E5Q65_RS15805 (3261428) 3261428..3262330 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T268754 WP_000897305.1 NZ_CP116480:3257673-3257984 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT268754 WP_000356397.1 NZ_CP116480:3257985-3258275 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References