Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3540820..3541490 Replicon chromosome
Accession NC_017522
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag CCDC5180_RS16835 Protein ID WP_003899954.1
Coordinates 3540820..3541164 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag CCDC5180_RS16840 Protein ID WP_003899955.1
Coordinates 3541161..3541490 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCDC5180_RS16810 3536707..3537996 + 1290 WP_003416640.1 ATP-binding protein -
CCDC5180_RS16825 3539700..3540134 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
CCDC5180_RS16830 3540137..3540589 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CCDC5180_RS16835 3540820..3541164 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CCDC5180_RS16840 3541161..3541490 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
CCDC5180_RS16845 3542028..3542375 + 348 WP_003899956.1 DUF2384 domain-containing protein -
CCDC5180_RS16850 3542372..3542992 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
CCDC5180_RS16855 3543152..3544417 - 1266 WP_003902423.1 hypothetical protein -
CCDC5180_RS16860 3544586..3544795 + 210 WP_003416778.1 hypothetical protein -
CCDC5180_RS16870 3545042..3546076 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3533178..3559248 26070


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T26875 WP_003899954.1 NC_017522:3540820-3541164 [Mycobacterium tuberculosis CCDC5180]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T26875 NC_017522:3540820-3541164 [Mycobacterium tuberculosis CCDC5180]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT26875 WP_003899955.1 NC_017522:3541161-3541490 [Mycobacterium tuberculosis CCDC5180]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT26875 NC_017522:3541161-3541490 [Mycobacterium tuberculosis CCDC5180]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References