Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 170823..171416 Replicon chromosome
Accession NZ_CP116478
Organism Pseudomonas syringae pv. actinidiae strain PSA.AH.01

Toxin (Protein)


Gene name graT Uniprot ID A0A2G7PD43
Locus tag PHA47_RS00820 Protein ID WP_003377943.1
Coordinates 170823..171101 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88B19
Locus tag PHA47_RS00825 Protein ID WP_005739893.1
Coordinates 171111..171416 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PHA47_RS00800 (PHA47_00800) 165975..166808 - 834 WP_005739898.1 transporter substrate-binding domain-containing protein -
PHA47_RS00805 (PHA47_00805) 166836..167708 - 873 WP_005613618.1 phytanoyl-CoA dioxygenase family protein -
PHA47_RS00810 (PHA47_00810) 167733..169253 - 1521 WP_024533279.1 amino acid ABC transporter permease/ATP-binding protein -
PHA47_RS00815 (PHA47_00815) 169738..170652 + 915 WP_019332092.1 LysR family transcriptional regulator -
PHA47_RS00820 (PHA47_00820) 170823..171101 + 279 WP_003377943.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PHA47_RS00825 (PHA47_00825) 171111..171416 + 306 WP_005739893.1 HigA family addiction module antitoxin Antitoxin
PHA47_RS00830 (PHA47_00830) 171450..171731 - 282 WP_017682769.1 accessory factor UbiK family protein -
PHA47_RS00835 (PHA47_00835) 172151..172489 + 339 WP_002555808.1 P-II family nitrogen regulator -
PHA47_RS00840 (PHA47_00840) 172524..173861 + 1338 WP_003377937.1 ammonium transporter -
PHA47_RS00845 (PHA47_00845) 174104..174529 + 426 WP_003377936.1 secondary thiamine-phosphate synthase enzyme YjbQ -
PHA47_RS00850 (PHA47_00850) 174628..174960 + 333 WP_003377934.1 transcriptional regulator SutA -
PHA47_RS00855 (PHA47_00855) 175011..176348 - 1338 WP_032685517.1 IS4-like element ISPsy33 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10512.18 Da        Isoelectric Point: 9.1490

>T268733 WP_003377943.1 NZ_CP116478:170823-171101 [Pseudomonas syringae pv. actinidiae]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEVLDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11158.01 Da        Isoelectric Point: 7.7509

>AT268733 WP_005739893.1 NZ_CP116478:171111-171416 [Pseudomonas syringae pv. actinidiae]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7PD43

Antitoxin

Source ID Structure
AlphaFold DB A0A0K8LYY6

References