Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4068581..4069122 Replicon chromosome
Accession NZ_CP116409
Organism Escherichia coli strain HH97

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag PL329_RS19945 Protein ID WP_000615983.1
Coordinates 4068844..4069122 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag PL329_RS19940 Protein ID WP_000729703.1
Coordinates 4068581..4068841 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PL329_RS19920 (4064158) 4064158..4064943 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
PL329_RS19925 (4064915) 4064915..4066627 + 1713 Protein_3890 flagellar biosynthesis protein FlhA -
PL329_RS19930 (4066949) 4066949..4067446 - 498 WP_000006259.1 REP-associated tyrosine transposase RayT -
PL329_RS19935 (4067622) 4067622..4068371 - 750 WP_271711327.1 C40 family peptidase -
PL329_RS19940 (4068581) 4068581..4068841 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PL329_RS19945 (4068844) 4068844..4069122 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PL329_RS19950 (4069278) 4069278..4070018 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PL329_RS19955 (4069989) 4069989..4070756 - 768 WP_000333380.1 class II glutamine amidotransferase -
PL329_RS19960 (4070962) 4070962..4071540 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4066949..4081016 14067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T268674 WP_000615983.1 NZ_CP116409:4068844-4069122 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT268674 WP_000729703.1 NZ_CP116409:4068581-4068841 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References