Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 61843..62570 | Replicon | plasmid pHH107-3 |
| Accession | NZ_CP116408 | ||
| Organism | Escherichia coli strain HH107 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A663AUB1 |
| Locus tag | PL328_RS24870 | Protein ID | WP_000558568.1 |
| Coordinates | 62259..62570 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | PL328_RS24865 | Protein ID | WP_000990392.1 |
| Coordinates | 61843..62262 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PL328_RS24835 (PL328_24835) | 57796..58281 | + | 486 | WP_000045622.1 | conjugative transfer protein MobI(A/C) | - |
| PL328_RS24840 (PL328_24840) | 58278..58643 | + | 366 | WP_001515713.1 | hypothetical protein | - |
| PL328_RS24845 (PL328_24845) | 58744..59724 | - | 981 | WP_016236480.1 | IS5-like element ISKpn26 family transposase | - |
| PL328_RS24850 (PL328_24850) | 59957..60106 | + | 150 | WP_232482723.1 | recombinase family protein | - |
| PL328_RS24855 (PL328_24855) | 60153..60857 | + | 705 | WP_061872725.1 | IS6-like element IS26 family transposase | - |
| PL328_RS24860 (PL328_24860) | 60994..61806 | + | 813 | WP_265894992.1 | hypothetical protein | - |
| PL328_RS24865 (PL328_24865) | 61843..62262 | - | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| PL328_RS24870 (PL328_24870) | 62259..62570 | - | 312 | WP_000558568.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| PL328_RS24875 (PL328_24875) | 62800..65808 | - | 3009 | WP_000718520.1 | Tn3 family transposase | - |
| PL328_RS24880 (PL328_24880) | 65967..66128 | + | 162 | Protein_64 | recombinase family protein | - |
| PL328_RS24885 (PL328_24885) | 66182..66886 | - | 705 | WP_061872725.1 | IS6-like element IS26 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..74050 | 74050 | |
| - | inside | IScluster/Tn | - | - | 42941..66886 | 23945 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12372.11 Da Isoelectric Point: 10.0935
>T268662 WP_000558568.1 NZ_CP116408:c62570-62259 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT268662 WP_000990392.1 NZ_CP116408:c62262-61843 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A663AUB1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |