Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2183221..2184089 Replicon chromosome
Accession NC_017522
Organism Mycobacterium tuberculosis CCDC5180

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag CCDC5180_RS10215 Protein ID WP_010886136.1
Coordinates 2183221..2183598 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag CCDC5180_RS10220 Protein ID WP_003409886.1
Coordinates 2183640..2184089 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCDC5180_RS10165 2179010..2179462 - 453 WP_003899095.1 lipoprotein -
CCDC5180_RS10170 2179526..2179927 + 402 WP_003409869.1 hypothetical protein -
CCDC5180_RS10175 2179920..2180102 - 183 WP_003409870.1 hypothetical protein -
CCDC5180_RS10180 2180216..2180566 - 351 WP_003409871.1 hypothetical protein -
CCDC5180_RS10185 2180577..2181479 - 903 WP_003899097.1 hypothetical protein -
CCDC5180_RS10190 2181500..2181691 - 192 WP_003409876.1 hypothetical protein -
CCDC5180_RS10195 2181692..2181988 - 297 WP_003409877.1 hypothetical protein -
CCDC5180_RS10200 2182228..2182443 + 216 WP_003409878.1 antitoxin -
CCDC5180_RS10205 2182440..2182751 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
CCDC5180_RS22605 2182725..2183246 - 522 WP_003904745.1 hypothetical protein -
CCDC5180_RS10215 2183221..2183598 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
CCDC5180_RS10220 2183640..2184089 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
CCDC5180_RS10225 2184086..2184631 + 546 WP_003409891.1 SecB-like chaperone -
CCDC5180_RS10230 2184520..2185134 - 615 WP_003901296.1 hypothetical protein -
CCDC5180_RS10235 2185183..2185479 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
CCDC5180_RS10240 2185476..2185727 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
CCDC5180_RS10245 2185714..2186208 + 495 WP_003899099.1 hypothetical protein -
CCDC5180_RS10250 2186368..2186775 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
CCDC5180_RS10255 2186779..2187051 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CCDC5180_RS10260 2187084..2188304 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T26851 WP_010886136.1 NC_017522:2183221-2183598 [Mycobacterium tuberculosis CCDC5180]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T26851 NC_017522:2183221-2183598 [Mycobacterium tuberculosis CCDC5180]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT26851 WP_003409886.1 NC_017522:2183640-2184089 [Mycobacterium tuberculosis CCDC5180]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT26851 NC_017522:2183640-2184089 [Mycobacterium tuberculosis CCDC5180]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References