Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 44613..45340 | Replicon | plasmid p2562_2 |
| Accession | NZ_CP116216 | ||
| Organism | Klebsiella michiganensis strain 2563 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A5Q9LNL2 |
| Locus tag | PHA56_RS30225 | Protein ID | WP_004118600.1 |
| Coordinates | 44613..44924 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | PHA56_RS30230 | Protein ID | WP_000990392.1 |
| Coordinates | 44921..45340 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PHA56_RS30205 (PHA56_30205) | 41127..41846 | + | 720 | WP_001102107.1 | (Fe-S)-binding protein | - |
| PHA56_RS30210 (PHA56_30210) | 41857..43284 | + | 1428 | WP_000023895.1 | LutB/LldF family L-lactate oxidation iron-sulfur protein | - |
| PHA56_RS30215 (PHA56_30215) | 43277..43972 | + | 696 | WP_004118595.1 | lactate utilization protein C | - |
| PHA56_RS30220 (PHA56_30220) | 44022..44402 | - | 381 | Protein_43 | gluconate permease | - |
| PHA56_RS30225 (PHA56_30225) | 44613..44924 | + | 312 | WP_004118600.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| PHA56_RS30230 (PHA56_30230) | 44921..45340 | + | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| PHA56_RS30235 (PHA56_30235) | 45377..46579 | - | 1203 | WP_003031541.1 | hypothetical protein | - |
| PHA56_RS30240 (PHA56_30240) | 46572..46901 | - | 330 | WP_004118613.1 | hypothetical protein | - |
| PHA56_RS30245 (PHA56_30245) | 46898..47557 | - | 660 | WP_000078540.1 | hypothetical protein | - |
| PHA56_RS30250 (PHA56_30250) | 47981..48289 | - | 309 | WP_020324596.1 | Ref family recombination enhancement nuclease | - |
| PHA56_RS30255 (PHA56_30255) | 48457..48597 | - | 141 | WP_004118614.1 | hypothetical protein | - |
| PHA56_RS30260 (PHA56_30260) | 48890..49243 | + | 354 | WP_001114073.1 | As(III)-sensing metalloregulatory transcriptional repressor ArsR | - |
| PHA56_RS30265 (PHA56_30265) | 49291..49653 | + | 363 | WP_000783215.1 | arsenite efflux transporter metallochaperone ArsD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..183276 | 183276 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12386.14 Da Isoelectric Point: 10.0935
>T268463 WP_004118600.1 NZ_CP116216:44613-44924 [Klebsiella michiganensis]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT268463 WP_000990392.1 NZ_CP116216:44921-45340 [Klebsiella michiganensis]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Q9LNL2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |