268407

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4035414..4036141 Replicon chromosome
Accession NZ_CP116194
Organism Escherichia coli strain DETEC-E1005

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag PIB63_RS19660 Protein ID WP_000550189.1
Coordinates 4035827..4036141 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PIB63_RS19655 Protein ID WP_000560266.1
Coordinates 4035414..4035830 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB63_RS19645 (4030574) 4030574..4032925 + 2352 WP_000695487.1 alpha-glucosidase -
PIB63_RS19650 (4033351) 4033351..4035369 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
PIB63_RS19655 (4035414) 4035414..4035830 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
PIB63_RS19660 (4035827) 4035827..4036141 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
PIB63_RS19665 (4036425) 4036425..4037561 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
PIB63_RS19670 (4037646) 4037646..4038149 + 504 WP_001333820.1 M48 family metallopeptidase -
PIB63_RS19675 (4038226) 4038226..4038918 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
PIB63_RS19680 (4038997) 4038997..4039983 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T268407 WP_000550189.1 NZ_CP116194:c4036141-4035827 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT268407 WP_000560266.1 NZ_CP116194:c4035830-4035414 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References