Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1003358..1004052 Replicon chromosome
Accession NZ_CP116194
Organism Escherichia coli strain DETEC-E1005

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag PIB63_RS04845 Protein ID WP_001263500.1
Coordinates 1003654..1004052 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIB63_RS04840 Protein ID WP_000554758.1
Coordinates 1003358..1003651 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB63_RS04815 (999175) 999175..999642 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
PIB63_RS04820 (999662) 999662..1000378 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
PIB63_RS04825 (1000391) 1000391..1001254 + 864 WP_001169518.1 flagellar motor stator protein MotA -
PIB63_RS04830 (1001257) 1001257..1002180 + 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
PIB63_RS04835 (1002251) 1002251..1003306 + 1056 WP_001226168.1 DNA polymerase IV -
PIB63_RS04840 (1003358) 1003358..1003651 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIB63_RS04845 (1003654) 1003654..1004052 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIB63_RS04850 (1004062) 1004062..1004514 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
PIB63_RS04855 (1004704) 1004704..1005843 + 1140 WP_000521561.1 RNA ligase RtcB family protein -
PIB63_RS04860 (1005840) 1005840..1006454 + 615 WP_000602124.1 peptide chain release factor H -
PIB63_RS04865 (1006511) 1006511..1007968 - 1458 WP_271291445.1 cytosol nonspecific dipeptidase -
PIB63_RS04870 (1008229) 1008229..1008687 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T268395 WP_001263500.1 NZ_CP116194:1003654-1004052 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT268395 WP_000554758.1 NZ_CP116194:1003358-1003651 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References