Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2270862..2271424 Replicon chromosome
Accession NZ_CP116191
Organism Escherichia coli strain DETEC-E1070

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag PIC72_RS11195 Protein ID WP_000605675.1
Coordinates 2271146..2271424 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag PIC72_RS11190 Protein ID WP_000781370.1
Coordinates 2270862..2271146 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC72_RS11175 (2266043) 2266043..2269090 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
PIC72_RS11180 (2269103) 2269103..2269987 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
PIC72_RS11185 (2269980) 2269980..2270633 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
PIC72_RS11190 (2270862) 2270862..2271146 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
PIC72_RS11195 (2271146) 2271146..2271424 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC72_RS11200 (2271610) 2271610..2272620 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
PIC72_RS11205 (2272754) 2272754..2274451 - 1698 WP_000433460.1 malate dehydrogenase -
PIC72_RS11210 (2274608) 2274608..2274745 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
PIC72_RS11215 (2274847) 2274847..2275062 - 216 WP_000495766.1 biofilm-dependent modulation protein -
PIC72_RS11220 (2275407) 2275407..2275838 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T268371 WP_000605675.1 NZ_CP116191:c2271424-2271146 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT268371 WP_000781370.1 NZ_CP116191:c2271146-2270862 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References