Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1231085..1231764 Replicon chromosome
Accession NZ_CP116191
Organism Escherichia coli strain DETEC-E1070

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag PIC72_RS05900 Protein ID WP_000057523.1
Coordinates 1231085..1231387 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag PIC72_RS05905 Protein ID WP_000806442.1
Coordinates 1231423..1231764 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC72_RS05880 (1226348) 1226348..1227568 - 1221 WP_001773866.1 fosmidomycin MFS transporter -
PIC72_RS05885 (1227786) 1227786..1229438 + 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
PIC72_RS05890 (1229475) 1229475..1229954 - 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
PIC72_RS05895 (1230158) 1230158..1230952 - 795 WP_001538398.1 TraB/GumN family protein -
PIC72_RS05900 (1231085) 1231085..1231387 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC72_RS05905 (1231423) 1231423..1231764 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
PIC72_RS05910 (1231822) 1231822..1234326 - 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
PIC72_RS05915 (1234588) 1234588..1235520 + 933 WP_001538399.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T268370 WP_000057523.1 NZ_CP116191:1231085-1231387 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT268370 WP_000806442.1 NZ_CP116191:1231423-1231764 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References