Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1003348..1004042 Replicon chromosome
Accession NZ_CP116191
Organism Escherichia coli strain DETEC-E1070

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag PIC72_RS04845 Protein ID WP_001263500.1
Coordinates 1003644..1004042 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIC72_RS04840 Protein ID WP_000554758.1
Coordinates 1003348..1003641 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC72_RS04815 (999165) 999165..999632 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
PIC72_RS04820 (999652) 999652..1000368 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
PIC72_RS04825 (1000381) 1000381..1001244 + 864 WP_001169518.1 flagellar motor stator protein MotA -
PIC72_RS04830 (1001247) 1001247..1002170 + 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
PIC72_RS04835 (1002241) 1002241..1003296 + 1056 WP_001226168.1 DNA polymerase IV -
PIC72_RS04840 (1003348) 1003348..1003641 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIC72_RS04845 (1003644) 1003644..1004042 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIC72_RS04850 (1004052) 1004052..1004504 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
PIC72_RS04855 (1004694) 1004694..1005833 + 1140 WP_000521561.1 RNA ligase RtcB family protein -
PIC72_RS04860 (1005830) 1005830..1006444 + 615 WP_000602124.1 peptide chain release factor H -
PIC72_RS04865 (1006501) 1006501..1007958 - 1458 WP_271291445.1 cytosol nonspecific dipeptidase -
PIC72_RS04870 (1008219) 1008219..1008677 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T268368 WP_001263500.1 NZ_CP116191:1003644-1004042 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT268368 WP_000554758.1 NZ_CP116191:1003348-1003641 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References