Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4115706..4116433 Replicon chromosome
Accession NZ_CP116188
Organism Escherichia coli strain DETEC-E223

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag PIC88_RS20135 Protein ID WP_000550189.1
Coordinates 4116119..4116433 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PIC88_RS20130 Protein ID WP_000560266.1
Coordinates 4115706..4116122 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC88_RS20120 (4110866) 4110866..4113217 + 2352 WP_000695487.1 alpha-glucosidase -
PIC88_RS20125 (4113643) 4113643..4115661 + 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
PIC88_RS20130 (4115706) 4115706..4116122 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
PIC88_RS20135 (4116119) 4116119..4116433 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
PIC88_RS20140 (4116717) 4116717..4117853 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
PIC88_RS20145 (4117938) 4117938..4118441 + 504 WP_001333820.1 M48 family metallopeptidase -
PIC88_RS20150 (4118518) 4118518..4119210 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
PIC88_RS20155 (4119289) 4119289..4120275 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T268349 WP_000550189.1 NZ_CP116188:c4116433-4116119 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT268349 WP_000560266.1 NZ_CP116188:c4116122-4115706 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References