Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 904416..905110 Replicon chromosome
Accession NZ_CP116188
Organism Escherichia coli strain DETEC-E223

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag PIC88_RS04345 Protein ID WP_001263489.1
Coordinates 904712..905110 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIC88_RS04340 Protein ID WP_000554758.1
Coordinates 904416..904709 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC88_RS04320 (900048) 900048..900545 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
PIC88_RS04325 (900769) 900769..902481 - 1713 Protein_844 flagellar biosynthesis protein FlhA -
PIC88_RS04330 (902453) 902453..903238 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
PIC88_RS04335 (903309) 903309..904364 + 1056 WP_001226164.1 DNA polymerase IV -
PIC88_RS04340 (904416) 904416..904709 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIC88_RS04345 (904712) 904712..905110 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIC88_RS04350 (905120) 905120..905572 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
PIC88_RS04355 (905890) 905890..906096 + 207 Protein_850 RtcB family protein -
PIC88_RS04360 (906092) 906092..906613 + 522 Protein_851 peptide chain release factor H -
PIC88_RS04365 (906670) 906670..908127 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
PIC88_RS04370 (908388) 908388..908846 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (909442) 909442..909522 + 81 NuclAT_9 - -
- (909442) 909442..909522 + 81 NuclAT_9 - -
- (909442) 909442..909522 + 81 NuclAT_9 - -
- (909442) 909442..909522 + 81 NuclAT_9 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T268335 WP_001263489.1 NZ_CP116188:904712-905110 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT268335 WP_000554758.1 NZ_CP116188:904416-904709 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References