Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4074412..4075211 Replicon chromosome
Accession NZ_CP116176
Organism Escherichia coli strain DETEC-E601

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag PIC02_RS19710 Protein ID WP_000347267.1
Coordinates 4074747..4075211 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PIC02_RS19705 Protein ID WP_001307405.1
Coordinates 4074412..4074747 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC02_RS19690 (4070197) 4070197..4070967 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
PIC02_RS19695 (4070983) 4070983..4072317 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
PIC02_RS19700 (4072692) 4072692..4074263 + 1572 WP_001273741.1 galactarate dehydratase -
PIC02_RS19705 (4074412) 4074412..4074747 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PIC02_RS19710 (4074747) 4074747..4075211 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PIC02_RS19715 (4075266) 4075266..4076075 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
PIC02_RS19720 (4076324) 4076324..4077604 + 1281 WP_236929999.1 tagatose-bisphosphate aldolase subunit KbaZ -
PIC02_RS19725 (4077627) 4077627..4078100 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
PIC02_RS19730 (4078111) 4078111..4078890 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
PIC02_RS19735 (4078880) 4078880..4079758 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
PIC02_RS19740 (4079776) 4079776..4080210 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4056125..4075211 19086


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T268297 WP_000347267.1 NZ_CP116176:4074747-4075211 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT268297 WP_001307405.1 NZ_CP116176:4074412-4074747 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References