Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3675803..3676344 Replicon chromosome
Accession NZ_CP116169
Organism Escherichia coli strain DETEC-P1056

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag PIC86_RS17875 Protein ID WP_000615976.1
Coordinates 3676066..3676344 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PIC86_RS17870 Protein ID WP_000729704.1
Coordinates 3675803..3676063 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC86_RS17845 (3671586) 3671586..3672371 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
PIC86_RS17850 (3672343) 3672343..3674055 + 1713 Protein_3491 flagellar biosynthesis protein FlhA -
PIC86_RS17855 (3674160) 3674160..3674438 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
PIC86_RS17860 (3674431) 3674431..3674787 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
PIC86_RS17865 (3674844) 3674844..3675617 - 774 WP_000543895.1 C40 family peptidase -
PIC86_RS17870 (3675803) 3675803..3676063 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PIC86_RS17875 (3676066) 3676066..3676344 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PIC86_RS17880 (3676500) 3676500..3677240 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PIC86_RS17885 (3677211) 3677211..3677978 - 768 WP_000333380.1 class II glutamine amidotransferase -
PIC86_RS17890 (3678184) 3678184..3678762 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 3674431..3692329 17898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T268274 WP_000615976.1 NZ_CP116169:3676066-3676344 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT268274 WP_000729704.1 NZ_CP116169:3675803-3676063 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References