Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1102298..1103025 Replicon chromosome
Accession NZ_CP116166
Organism Escherichia coli strain DETEC-P169

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag PID12_RS05395 Protein ID WP_000547564.1
Coordinates 1102298..1102609 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PID12_RS05400 Protein ID WP_000126294.1
Coordinates 1102606..1103025 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PID12_RS05365 (1097440) 1097440..1099149 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
PID12_RS05370 (1099159) 1099159..1099701 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
PID12_RS05375 (1099701) 1099701..1100468 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
PID12_RS05380 (1100465) 1100465..1100875 + 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
PID12_RS05385 (1100868) 1100868..1101338 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
PID12_RS05390 (1101363) 1101363..1102124 + 762 WP_001026446.1 hypothetical protein -
PID12_RS05395 (1102298) 1102298..1102609 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
PID12_RS05400 (1102606) 1102606..1103025 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
PID12_RS05405 (1103139) 1103139..1104563 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
PID12_RS05410 (1104572) 1104572..1106029 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
PID12_RS05415 (1106289) 1106289..1107299 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
PID12_RS05420 (1107448) 1107448..1107975 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T268238 WP_000547564.1 NZ_CP116166:1102298-1102609 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT268238 WP_000126294.1 NZ_CP116166:1102606-1103025 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References