Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3727427..3728121 Replicon chromosome
Accession NZ_CP116159
Organism Escherichia coli strain DETEC-P351

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag PIC26_RS18000 Protein ID WP_001263489.1
Coordinates 3727427..3727825 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIC26_RS18005 Protein ID WP_000554758.1
Coordinates 3727828..3728121 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3723015) 3723015..3723095 - 81 NuclAT_11 - -
- (3723015) 3723015..3723095 - 81 NuclAT_11 - -
- (3723015) 3723015..3723095 - 81 NuclAT_11 - -
- (3723015) 3723015..3723095 - 81 NuclAT_11 - -
PIC26_RS17975 (3723691) 3723691..3724149 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
PIC26_RS17980 (3724410) 3724410..3725867 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
PIC26_RS17985 (3725924) 3725924..3726445 - 522 Protein_3518 peptide chain release factor H -
PIC26_RS17990 (3726441) 3726441..3726647 - 207 Protein_3519 RtcB family protein -
PIC26_RS17995 (3726965) 3726965..3727417 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
PIC26_RS18000 (3727427) 3727427..3727825 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIC26_RS18005 (3727828) 3727828..3728121 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIC26_RS18010 (3728173) 3728173..3729228 - 1056 WP_001226164.1 DNA polymerase IV -
PIC26_RS18015 (3729299) 3729299..3730084 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
PIC26_RS18020 (3730056) 3730056..3731768 + 1713 Protein_3525 flagellar biosynthesis protein FlhA -
PIC26_RS18025 (3731992) 3731992..3732489 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3701022..3742707 41685


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T268217 WP_001263489.1 NZ_CP116159:c3727825-3727427 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT268217 WP_000554758.1 NZ_CP116159:c3728121-3727828 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References