Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4814258..4814860 Replicon chromosome
Accession NZ_CP116136
Organism Escherichia coli strain DETEC-P649

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag PIB66_RS22830 Protein ID WP_000897305.1
Coordinates 4814549..4814860 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PIB66_RS22825 Protein ID WP_000356395.1
Coordinates 4814258..4814548 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB66_RS22790 (4809882) 4809882..4810784 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
PIB66_RS22795 (4810781) 4810781..4811416 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
PIB66_RS22800 (4811413) 4811413..4812342 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
PIB66_RS22805 (4812524) 4812524..4812766 - 243 WP_001309881.1 CopG family transcriptional regulator -
PIB66_RS22810 (4812985) 4812985..4813203 - 219 WP_001295676.1 CopG family transcriptional regulator -
PIB66_RS22815 (4813622) 4813622..4813900 - 279 WP_001315112.1 hypothetical protein -
PIB66_RS22820 (4813952) 4813952..4814173 - 222 WP_001550354.1 hypothetical protein -
PIB66_RS22825 (4814258) 4814258..4814548 - 291 WP_000356395.1 NadS family protein Antitoxin
PIB66_RS22830 (4814549) 4814549..4814860 - 312 WP_000897305.1 hypothetical protein Toxin
PIB66_RS22835 (4815089) 4815089..4815997 + 909 WP_001550353.1 alpha/beta hydrolase -
PIB66_RS22840 (4816165) 4816165..4817079 - 915 WP_109553727.1 transposase -
PIB66_RS22845 (4817092) 4817092..4817979 - 888 Protein_4463 hypothetical protein -
PIB66_RS22850 (4818395) 4818395..4819336 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
PIB66_RS22855 (4819381) 4819381..4819818 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T268140 WP_000897305.1 NZ_CP116136:c4814860-4814549 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT268140 WP_000356395.1 NZ_CP116136:c4814548-4814258 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References