Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1222807..1223534 Replicon chromosome
Accession NZ_CP116136
Organism Escherichia coli strain DETEC-P649

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag PIB66_RS05925 Protein ID WP_000547555.1
Coordinates 1222807..1223118 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PIB66_RS05930 Protein ID WP_000126294.1
Coordinates 1223115..1223534 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB66_RS05900 (1218742) 1218742..1220451 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
PIB66_RS05905 (1220461) 1220461..1221003 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
PIB66_RS05910 (1221003) 1221003..1221770 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
PIB66_RS05915 (1221767) 1221767..1222177 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
PIB66_RS05920 (1222170) 1222170..1222640 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
PIB66_RS05925 (1222807) 1222807..1223118 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
PIB66_RS05930 (1223115) 1223115..1223534 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
PIB66_RS05935 (1223613) 1223613..1225037 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
PIB66_RS05940 (1225046) 1225046..1226503 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
PIB66_RS05945 (1226763) 1226763..1227773 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
PIB66_RS05950 (1227922) 1227922..1228449 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T268124 WP_000547555.1 NZ_CP116136:1222807-1223118 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT268124 WP_000126294.1 NZ_CP116136:1223115-1223534 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References