Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 8905..9551 Replicon plasmid pDETEC34
Accession NZ_CP116126
Organism Escherichia coli strain DETEC-P666

Toxin (Protein)


Gene name higB Uniprot ID A0A3P1YQM6
Locus tag PIB57_RS24960 Protein ID WP_025667955.1
Coordinates 9204..9551 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1V3UZT0
Locus tag PIB57_RS24955 Protein ID WP_001259436.1
Coordinates 8905..9204 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB57_RS24920 (PIB57_24925) 4008..4256 + 249 WP_000730008.1 hypothetical protein -
PIB57_RS24925 (PIB57_24930) 5387..5578 + 192 WP_000183351.1 hypothetical protein -
PIB57_RS24930 (PIB57_24935) 5575..5922 + 348 Protein_10 ORF6N domain-containing protein -
PIB57_RS24935 (PIB57_24940) 6091..6717 + 627 WP_231531872.1 Rha family transcriptional regulator -
PIB57_RS24940 (PIB57_24945) 6752..7387 - 636 WP_032154031.1 hypothetical protein -
PIB57_RS24945 (PIB57_24950) 8044..8295 - 252 WP_032154030.1 helix-turn-helix domain-containing protein -
PIB57_RS24950 (PIB57_24955) 8361..8741 - 381 WP_032154029.1 hypothetical protein -
PIB57_RS24955 (PIB57_24960) 8905..9204 - 300 WP_001259436.1 XRE family transcriptional regulator Antitoxin
PIB57_RS24960 (PIB57_24965) 9204..9551 - 348 WP_025667955.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIB57_RS24965 (PIB57_24970) 9796..10059 - 264 WP_000424604.1 hypothetical protein -
PIB57_RS24970 (PIB57_24975) 10083..10370 - 288 WP_000356589.1 hypothetical protein -
PIB57_RS24975 (PIB57_24980) 11014..11991 - 978 WP_001420765.1 plasmid replication initiator RepA -
PIB57_RS24980 (PIB57_24985) 12203..12295 + 93 Protein_20 hypothetical protein -
PIB57_RS24985 (PIB57_24990) 12458..13021 + 564 WP_001523082.1 serine acetyltransferase -
PIB57_RS24990 (PIB57_24995) 12985..13602 - 618 WP_001523080.1 tail fiber assembly protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..47568 47568


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-80)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13530.50 Da        Isoelectric Point: 9.3634

>T268115 WP_025667955.1 NZ_CP116126:c9551-9204 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Download         Length: 348 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11074.92 Da        Isoelectric Point: 5.2090

>AT268115 WP_001259436.1 NZ_CP116126:c9204-8905 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P1YQM6

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3UZT0

References