Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1373146..1373710 Replicon chromosome
Accession NC_017474
Organism Lactobacillus casei BD-II

Toxin (Protein)


Gene name yafQ Uniprot ID A0A6G9EML4
Locus tag LCBD_RS07075 Protein ID WP_014951859.1
Coordinates 1373408..1373710 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag LCBD_RS07070 Protein ID WP_003570051.1
Coordinates 1373146..1373415 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCBD_RS07040 1368150..1369037 - 888 WP_012491506.1 ABC transporter ATP-binding protein -
LCBD_RS07045 1369235..1370461 - 1227 WP_012491507.1 hypothetical protein -
LCBD_RS07050 1370659..1371063 + 405 WP_012491508.1 MerR family transcriptional regulator -
LCBD_RS07055 1371056..1371382 + 327 WP_012491509.1 YrdB family protein -
LCBD_RS07060 1371445..1372074 + 630 WP_012491510.1 DUF2399 domain-containing protein -
LCBD_RS07065 1372119..1372772 + 654 WP_012491511.1 GNAT family N-acetyltransferase -
LCBD_RS07070 1373146..1373415 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
LCBD_RS07075 1373408..1373710 + 303 WP_014951859.1 type II toxin-antitoxin system YafQ family toxin Toxin
LCBD_RS17250 1374049..1374192 + 144 WP_003574774.1 hypothetical protein -
LCBD_RS07080 1374310..1375002 + 693 WP_012491513.1 hypothetical protein -
LCBD_RS07085 1375238..1377415 + 2178 WP_012491514.1 RNA-binding transcriptional accessory protein -
LCBD_RS07090 1377480..1378409 - 930 WP_003599410.1 IS30-like element ISLpl1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1377480..1378409 929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11441.27 Da        Isoelectric Point: 10.2818

>T26810 WP_014951859.1 NC_017474:1373408-1373710 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T26810 NC_017474:1373408-1373710 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTCATTTTGACCCTGGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT26810 WP_003570051.1 NC_017474:1373146-1373415 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT26810 NC_017474:1373146-1373415 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACTATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G9EML4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References