Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1342257..1342821 Replicon chromosome
Accession NC_017473
Organism Lactobacillus casei LC2W

Toxin (Protein)


Gene name yafQ Uniprot ID A0A6G9EML4
Locus tag LC2W_RS06845 Protein ID WP_014951859.1
Coordinates 1342519..1342821 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag LC2W_RS06840 Protein ID WP_003570051.1
Coordinates 1342257..1342526 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC2W_RS06810 1337261..1338148 - 888 WP_024109452.1 ABC transporter ATP-binding protein -
LC2W_RS06815 1338346..1339572 - 1227 WP_012491507.1 hypothetical protein -
LC2W_RS06820 1339770..1340174 + 405 WP_012491508.1 MerR family transcriptional regulator -
LC2W_RS06825 1340167..1340493 + 327 WP_012491509.1 YrdB family protein -
LC2W_RS06830 1340556..1341185 + 630 WP_012491510.1 DUF2399 domain-containing protein -
LC2W_RS06835 1341230..1341883 + 654 WP_012491511.1 GNAT family N-acetyltransferase -
LC2W_RS06840 1342257..1342526 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
LC2W_RS06845 1342519..1342821 + 303 WP_014951859.1 type II toxin-antitoxin system YafQ family toxin Toxin
LC2W_RS16885 1343160..1343303 + 144 WP_003574774.1 hypothetical protein -
LC2W_RS06850 1343421..1344113 + 693 WP_012491513.1 hypothetical protein -
LC2W_RS06855 1344349..1346526 + 2178 WP_012491514.1 RNA-binding transcriptional accessory protein -
LC2W_RS06860 1346591..1347520 - 930 WP_003599410.1 IS30-like element ISLpl1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1346591..1347520 929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11441.27 Da        Isoelectric Point: 10.2818

>T26807 WP_014951859.1 NC_017473:1342519-1342821 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T26807 NC_017473:1342519-1342821 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTCATTTTGACCCTGGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT26807 WP_003570051.1 NC_017473:1342257-1342526 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT26807 NC_017473:1342257-1342526 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACTATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G9EML4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References