Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1004268..1004962 Replicon chromosome
Accession NZ_CP116115
Organism Escherichia coli strain DETEC-P793

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag PIC92_RS04860 Protein ID WP_001263500.1
Coordinates 1004564..1004962 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIC92_RS04855 Protein ID WP_000554758.1
Coordinates 1004268..1004561 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC92_RS04830 (1000085) 1000085..1000552 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
PIC92_RS04835 (1000572) 1000572..1001288 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
PIC92_RS04840 (1001301) 1001301..1002164 + 864 WP_001169518.1 flagellar motor stator protein MotA -
PIC92_RS04845 (1002167) 1002167..1003090 + 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
PIC92_RS04850 (1003161) 1003161..1004216 + 1056 WP_001226168.1 DNA polymerase IV -
PIC92_RS04855 (1004268) 1004268..1004561 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIC92_RS04860 (1004564) 1004564..1004962 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIC92_RS04865 (1004972) 1004972..1005424 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
PIC92_RS04870 (1005614) 1005614..1006753 + 1140 WP_000521561.1 RNA ligase RtcB family protein -
PIC92_RS04875 (1006750) 1006750..1007364 + 615 WP_000602124.1 peptide chain release factor H -
PIC92_RS04880 (1007421) 1007421..1008878 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
PIC92_RS04885 (1009139) 1009139..1009597 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T268061 WP_001263500.1 NZ_CP116115:1004564-1004962 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT268061 WP_000554758.1 NZ_CP116115:1004268-1004561 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References