Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3644142..3644836 Replicon chromosome
Accession NZ_CP116110
Organism Escherichia coli strain DETEC-P80

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag PIC84_RS18015 Protein ID WP_001263489.1
Coordinates 3644142..3644540 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIC84_RS18020 Protein ID WP_000554758.1
Coordinates 3644543..3644836 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3639730) 3639730..3639810 - 81 NuclAT_10 - -
- (3639730) 3639730..3639810 - 81 NuclAT_10 - -
- (3639730) 3639730..3639810 - 81 NuclAT_10 - -
- (3639730) 3639730..3639810 - 81 NuclAT_10 - -
PIC84_RS17990 (3640406) 3640406..3640864 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
PIC84_RS17995 (3641125) 3641125..3642582 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
PIC84_RS18000 (3642639) 3642639..3643160 - 522 Protein_3520 peptide chain release factor H -
PIC84_RS18005 (3643156) 3643156..3643362 - 207 Protein_3521 RtcB family protein -
PIC84_RS18010 (3643680) 3643680..3644132 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
PIC84_RS18015 (3644142) 3644142..3644540 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIC84_RS18020 (3644543) 3644543..3644836 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIC84_RS18025 (3644888) 3644888..3645943 - 1056 WP_001226164.1 DNA polymerase IV -
PIC84_RS18030 (3646014) 3646014..3646799 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
PIC84_RS18035 (3646771) 3646771..3648483 + 1713 Protein_3527 flagellar biosynthesis protein FlhA -
PIC84_RS18040 (3648707) 3648707..3649204 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T268033 WP_001263489.1 NZ_CP116110:c3644540-3644142 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT268033 WP_000554758.1 NZ_CP116110:c3644836-3644543 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References