Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3777823..3778364 Replicon chromosome
Accession NZ_CP116106
Organism Escherichia coli strain DETEC-P829

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag PIC05_RS18415 Protein ID WP_000615976.1
Coordinates 3778086..3778364 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PIC05_RS18410 Protein ID WP_000729704.1
Coordinates 3777823..3778083 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC05_RS18385 (3773606) 3773606..3774391 - 786 WP_000207590.1 putative lateral flagellar export/assembly protein LafU -
PIC05_RS18390 (3774363) 3774363..3776075 + 1713 Protein_3597 flagellar biosynthesis protein FlhA -
PIC05_RS18395 (3776180) 3776180..3776458 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
PIC05_RS18400 (3776451) 3776451..3776807 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
PIC05_RS18405 (3776864) 3776864..3777637 - 774 WP_000543895.1 C40 family peptidase -
PIC05_RS18410 (3777823) 3777823..3778083 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PIC05_RS18415 (3778086) 3778086..3778364 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PIC05_RS18420 (3778520) 3778520..3779260 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PIC05_RS18425 (3779231) 3779231..3779998 - 768 WP_000333380.1 class II glutamine amidotransferase -
PIC05_RS18430 (3780204) 3780204..3780782 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 3776451..3795001 18550


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T267998 WP_000615976.1 NZ_CP116106:3778086-3778364 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT267998 WP_000729704.1 NZ_CP116106:3777823-3778083 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References