Detailed information of TA system TA267980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4934758..4935360
• Replicon: chromosome
• Accession: NZ_CP116103
• Organism: Escherichia coli strain DETEC-P836

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: PIB67_RS24005
• Protein ID: WP_000897302.1
• Coordinates: 4934758..4935069 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PIB67_RS24010
• Protein ID: WP_000356397.1
• Coordinates: 4935070..4935360 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PIB67_RS23975 (4929788)	4929788..4930573	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
PIB67_RS23980 (4930672)	4930672..4931271	+	600	WP_001314324.1	glucose-1-phosphatase	-
PIB67_RS23985 (4931265)	4931265..4932137	+	873	WP_000920762.1	virulence factor BrkB family protein	-
PIB67_RS23990 (4932134)	4932134..4932571	+	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
PIB67_RS23995 (4932616)	4932616..4933557	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
PIB67_RS24000 (4933621)	4933621..4934529	-	909	WP_001774092.1	alpha/beta hydrolase	-
PIB67_RS24005 (4934758)	4934758..4935069	+	312	WP_000897302.1	hypothetical protein	Toxin
PIB67_RS24010 (4935070)	4935070..4935360	+	291	WP_000356397.1	NadS family protein	Antitoxin
PIB67_RS24015 (4935719)	4935719..4935997	+	279	WP_001296612.1	hypothetical protein	-
PIB67_RS24020 (4936393)	4936393..4936611	+	219	WP_001314326.1	CopG family transcriptional regulator	-
PIB67_RS24025 (4936827)	4936827..4937756	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
PIB67_RS24030 (4937753)	4937753..4938388	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
PIB67_RS24035 (4938385)	4938385..4939287	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T267980 WP_000897302.1 NZ_CP116103:4934758-4935069 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T267980 NZ_CP116103:4934758-4935069 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT267980 WP_000356397.1 NZ_CP116103:4935070-4935360 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT267980 NZ_CP116103:4935070-4935360 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416