Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1003368..1004062 Replicon chromosome
Accession NZ_CP116103
Organism Escherichia coli strain DETEC-P836

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag PIB67_RS04840 Protein ID WP_001263500.1
Coordinates 1003664..1004062 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIB67_RS04835 Protein ID WP_000554758.1
Coordinates 1003368..1003661 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIB67_RS04810 (999185) 999185..999652 + 468 WP_000725261.1 flagellar basal body-associated FliL family protein -
PIB67_RS04815 (999672) 999672..1000388 + 717 WP_000938731.1 FliA/WhiG family RNA polymerase sigma factor -
PIB67_RS04820 (1000401) 1000401..1001264 + 864 WP_001169518.1 flagellar motor stator protein MotA -
PIB67_RS04825 (1001267) 1001267..1002190 + 924 WP_001532973.1 putative lateral flagellar export/assembly protein LafU -
PIB67_RS04830 (1002261) 1002261..1003316 + 1056 WP_001226168.1 DNA polymerase IV -
PIB67_RS04835 (1003368) 1003368..1003661 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIB67_RS04840 (1003664) 1003664..1004062 + 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIB67_RS04845 (1004072) 1004072..1004524 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
PIB67_RS04850 (1004714) 1004714..1005853 + 1140 WP_000521561.1 RNA ligase RtcB family protein -
PIB67_RS04855 (1005850) 1005850..1006464 + 615 WP_000602124.1 peptide chain release factor H -
PIB67_RS04860 (1006521) 1006521..1007978 - 1458 WP_271291445.1 cytosol nonspecific dipeptidase -
PIB67_RS04865 (1008239) 1008239..1008697 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T267966 WP_001263500.1 NZ_CP116103:1003664-1004062 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT267966 WP_000554758.1 NZ_CP116103:1003368-1003661 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References