Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1650049..1650728 Replicon chromosome
Accession NZ_CP116100
Organism Escherichia coli strain DETEC-P881

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag PIC52_RS08220 Protein ID WP_000057523.1
Coordinates 1650426..1650728 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag PIC52_RS08215 Protein ID WP_000806442.1
Coordinates 1650049..1650390 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC52_RS08205 (1646293) 1646293..1647225 - 933 WP_000883041.1 glutaminase A -
PIC52_RS08210 (1647487) 1647487..1649991 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
PIC52_RS08215 (1650049) 1650049..1650390 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
PIC52_RS08220 (1650426) 1650426..1650728 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC52_RS08225 (1650861) 1650861..1651655 + 795 WP_001526714.1 TraB/GumN family protein -
PIC52_RS08230 (1651859) 1651859..1652338 + 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
PIC52_RS08235 (1652375) 1652375..1654027 - 1653 WP_271287364.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
PIC52_RS08240 (1654245) 1654245..1655465 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T267935 WP_000057523.1 NZ_CP116100:c1650728-1650426 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT267935 WP_000806442.1 NZ_CP116100:c1650390-1650049 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References