Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 651892..652454 Replicon chromosome
Accession NZ_CP116100
Organism Escherichia coli strain DETEC-P881

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag PIC52_RS03210 Protein ID WP_000605675.1
Coordinates 651892..652170 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag PIC52_RS03215 Protein ID WP_000781370.1
Coordinates 652170..652454 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC52_RS03185 (647480) 647480..647911 - 432 WP_000152305.1 peroxiredoxin OsmC -
PIC52_RS03190 (648255) 648255..648470 + 216 WP_000495774.1 biofilm-dependent modulation protein -
PIC52_RS03195 (648572) 648572..648709 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
PIC52_RS03200 (648865) 648865..650562 + 1698 WP_001607334.1 malate dehydrogenase -
PIC52_RS03205 (650696) 650696..651706 + 1011 WP_000642417.1 alcohol dehydrogenase AdhP -
PIC52_RS03210 (651892) 651892..652170 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC52_RS03215 (652170) 652170..652454 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
PIC52_RS03220 (652683) 652683..653336 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
PIC52_RS03225 (653329) 653329..654213 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
PIC52_RS03230 (654226) 654226..657273 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T267934 WP_000605675.1 NZ_CP116100:651892-652170 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT267934 WP_000781370.1 NZ_CP116100:652170-652454 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References