Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 848478..849172 Replicon chromosome
Accession NZ_CP116094
Organism Escherichia coli strain DETEC-S560

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag PIC78_RS04065 Protein ID WP_001263489.1
Coordinates 848774..849172 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIC78_RS04060 Protein ID WP_000554758.1
Coordinates 848478..848771 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC78_RS04040 (844110) 844110..844607 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
PIC78_RS04045 (844831) 844831..846543 - 1713 Protein_781 flagellar biosynthesis protein FlhA -
PIC78_RS04050 (846515) 846515..847300 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
PIC78_RS04055 (847371) 847371..848426 + 1056 WP_001226164.1 DNA polymerase IV -
PIC78_RS04060 (848478) 848478..848771 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIC78_RS04065 (848774) 848774..849172 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIC78_RS04070 (849182) 849182..849634 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
PIC78_RS04075 (849952) 849952..850158 + 207 Protein_787 RtcB family protein -
PIC78_RS04080 (850154) 850154..850675 + 522 Protein_788 peptide chain release factor H -
PIC78_RS04085 (850732) 850732..852189 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
PIC78_RS04090 (852450) 852450..852908 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (853504) 853504..853584 + 81 NuclAT_11 - -
- (853504) 853504..853584 + 81 NuclAT_11 - -
- (853504) 853504..853584 + 81 NuclAT_11 - -
- (853504) 853504..853584 + 81 NuclAT_11 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 833649..867460 33811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T267899 WP_001263489.1 NZ_CP116094:848774-849172 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT267899 WP_000554758.1 NZ_CP116094:848478-848771 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References