Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4167432..4167973 Replicon chromosome
Accession NZ_CP116088
Organism Escherichia coli strain DETEC-S565

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag PIC22_RS20290 Protein ID WP_000615982.1
Coordinates 4167432..4167710 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag PIC22_RS20295 Protein ID WP_000729704.1
Coordinates 4167713..4167973 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC22_RS20275 (4165014) 4165014..4165592 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
PIC22_RS20280 (4165798) 4165798..4166565 + 768 WP_000333380.1 class II glutamine amidotransferase -
PIC22_RS20285 (4166536) 4166536..4167276 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
PIC22_RS20290 (4167432) 4167432..4167710 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
PIC22_RS20295 (4167713) 4167713..4167973 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
PIC22_RS20300 (4168159) 4168159..4168932 + 774 WP_000543895.1 C40 family peptidase -
PIC22_RS20305 (4168989) 4168989..4169345 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
PIC22_RS20310 (4169338) 4169338..4169616 - 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
PIC22_RS20315 (4169721) 4169721..4171433 - 1713 Protein_3954 flagellar biosynthesis protein FlhA -
PIC22_RS20320 (4171405) 4171405..4172190 + 786 WP_000207557.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4158483..4169616 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T267880 WP_000615982.1 NZ_CP116088:c4167710-4167432 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T267880 NZ_CP116088:c4167710-4167432 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT267880 WP_000729704.1 NZ_CP116088:c4167973-4167713 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT267880 NZ_CP116088:c4167973-4167713 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References