Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2550581..2551380 Replicon chromosome
Accession NZ_CP116088
Organism Escherichia coli strain DETEC-S565

Toxin (Protein)


Gene name yhaV Uniprot ID A0A6H2GMC5
Locus tag PIC22_RS12630 Protein ID WP_000347279.1
Coordinates 2550916..2551380 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PIC22_RS12625 Protein ID WP_001307405.1
Coordinates 2550581..2550916 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC22_RS12610 (2546366) 2546366..2547136 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
PIC22_RS12615 (2547152) 2547152..2548486 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
PIC22_RS12620 (2548861) 2548861..2550432 + 1572 WP_001273741.1 galactarate dehydratase -
PIC22_RS12625 (2550581) 2550581..2550916 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PIC22_RS12630 (2550916) 2550916..2551380 + 465 WP_000347279.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PIC22_RS12635 (2551435) 2551435..2552244 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
PIC22_RS12640 (2552493) 2552493..2553773 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
PIC22_RS12645 (2553796) 2553796..2554269 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
PIC22_RS12650 (2554280) 2554280..2555059 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
PIC22_RS12655 (2555049) 2555049..2555927 + 879 WP_271291552.1 PTS N-acetylgalactosamine transporter subunit IID -
PIC22_RS12660 (2555945) 2555945..2556379 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2538646..2551380 12734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17869.24 Da        Isoelectric Point: 9.4947

>T267876 WP_000347279.1 NZ_CP116088:2550916-2551380 [Escherichia coli]
MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT267876 WP_001307405.1 NZ_CP116088:2550581-2550916 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GMC5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References