Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3639630..3640309 Replicon chromosome
Accession NZ_CP116085
Organism Escherichia coli strain DETEC-S566

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag PIC95_RS18200 Protein ID WP_000057523.1
Coordinates 3640007..3640309 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag PIC95_RS18195 Protein ID WP_000806442.1
Coordinates 3639630..3639971 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC95_RS18185 (3635874) 3635874..3636806 - 933 WP_000883041.1 glutaminase A -
PIC95_RS18190 (3637068) 3637068..3639572 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
PIC95_RS18195 (3639630) 3639630..3639971 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
PIC95_RS18200 (3640007) 3640007..3640309 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC95_RS18205 (3640442) 3640442..3641236 + 795 WP_000365147.1 TraB/GumN family protein -
PIC95_RS18210 (3641440) 3641440..3641919 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
PIC95_RS18215 (3641943) 3641943..3642743 + 801 WP_000439798.1 hypothetical protein -
PIC95_RS18220 (3642740) 3642740..3643243 + 504 WP_000667000.1 hypothetical protein -
PIC95_RS18225 (3643281) 3643281..3644933 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T267856 WP_000057523.1 NZ_CP116085:c3640309-3640007 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT267856 WP_000806442.1 NZ_CP116085:c3639971-3639630 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References