Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2551022..2551584 Replicon chromosome
Accession NZ_CP116074
Organism Escherichia coli strain DETEC-S586

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag PIC49_RS12245 Protein ID WP_000605676.1
Coordinates 2551022..2551300 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag PIC49_RS12250 Protein ID WP_000781370.1
Coordinates 2551300..2551584 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC49_RS12220 (2546608) 2546608..2547039 - 432 WP_000152305.1 peroxiredoxin OsmC -
PIC49_RS12225 (2547384) 2547384..2547599 + 216 WP_000495766.1 biofilm-dependent modulation protein -
PIC49_RS12230 (2547701) 2547701..2547838 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
PIC49_RS12235 (2547995) 2547995..2549692 + 1698 WP_000433464.1 malate dehydrogenase -
PIC49_RS12240 (2549826) 2549826..2550836 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
PIC49_RS12245 (2551022) 2551022..2551300 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC49_RS12250 (2551300) 2551300..2551584 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
PIC49_RS12255 (2551812) 2551812..2552465 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
PIC49_RS12260 (2552458) 2552458..2553342 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
PIC49_RS12265 (2553355) 2553355..2556402 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T267822 WP_000605676.1 NZ_CP116074:2551022-2551300 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT267822 WP_000781370.1 NZ_CP116074:2551300-2551584 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References