Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3987046..3987740 Replicon chromosome
Accession NZ_CP116071
Organism Escherichia coli strain DETEC-S589

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag PIC30_RS19935 Protein ID WP_001263500.1
Coordinates 3987046..3987444 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag PIC30_RS19940 Protein ID WP_000554758.1
Coordinates 3987447..3987740 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC30_RS19910 (3982411) 3982411..3982869 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
PIC30_RS19915 (3983130) 3983130..3984587 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
PIC30_RS19920 (3984644) 3984644..3985258 - 615 WP_000602123.1 peptide chain release factor H -
PIC30_RS19925 (3985255) 3985255..3986394 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
PIC30_RS19930 (3986584) 3986584..3987036 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
PIC30_RS19935 (3987046) 3987046..3987444 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
PIC30_RS19940 (3987447) 3987447..3987740 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
PIC30_RS19945 (3987792) 3987792..3988847 - 1056 WP_001226168.1 DNA polymerase IV -
PIC30_RS19950 (3988918) 3988918..3989703 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
PIC30_RS19955 (3989675) 3989675..3991387 + 1713 Protein_3911 flagellar biosynthesis protein FlhA -
PIC30_RS19960 (3991466) 3991466..3991624 + 159 WP_014639450.1 hypothetical protein -
PIC30_RS19965 (3991707) 3991707..3992204 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3987046..4007400 20354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T267795 WP_001263500.1 NZ_CP116071:c3987444-3987046 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT267795 WP_000554758.1 NZ_CP116071:c3987740-3987447 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References