Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3758133..3758812 Replicon chromosome
Accession NZ_CP116071
Organism Escherichia coli strain DETEC-S589

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag PIC30_RS18870 Protein ID WP_000057524.1
Coordinates 3758510..3758812 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag PIC30_RS18865 Protein ID WP_000806442.1
Coordinates 3758133..3758474 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC30_RS18855 (3754377) 3754377..3755309 - 933 WP_000883052.1 glutaminase A -
PIC30_RS18860 (3755571) 3755571..3758075 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
PIC30_RS18865 (3758133) 3758133..3758474 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
PIC30_RS18870 (3758510) 3758510..3758812 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC30_RS18875 (3758945) 3758945..3759739 + 795 WP_000365177.1 TraB/GumN family protein -
PIC30_RS18880 (3759943) 3759943..3760422 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
PIC30_RS18885 (3760590) 3760590..3761246 + 657 WP_001363258.1 hypothetical protein -
PIC30_RS18890 (3761243) 3761243..3761746 + 504 WP_000667000.1 hypothetical protein -
PIC30_RS18895 (3761784) 3761784..3763436 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3750822..3761746 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T267793 WP_000057524.1 NZ_CP116071:c3758812-3758510 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT267793 WP_000806442.1 NZ_CP116071:c3758474-3758133 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References