Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2602008..2602570 Replicon chromosome
Accession NZ_CP116071
Organism Escherichia coli strain DETEC-S589

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag PIC30_RS12790 Protein ID WP_000605675.1
Coordinates 2602008..2602286 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag PIC30_RS12795 Protein ID WP_000781370.1
Coordinates 2602286..2602570 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIC30_RS12765 (2597595) 2597595..2598026 - 432 WP_000152310.1 peroxiredoxin OsmC -
PIC30_RS12770 (2598371) 2598371..2598586 + 216 WP_000495766.1 biofilm-dependent modulation protein -
PIC30_RS12775 (2598688) 2598688..2598825 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
PIC30_RS12780 (2598981) 2598981..2600678 + 1698 WP_000433462.1 malate dehydrogenase -
PIC30_RS12785 (2600812) 2600812..2601822 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
PIC30_RS12790 (2602008) 2602008..2602286 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PIC30_RS12795 (2602286) 2602286..2602570 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
PIC30_RS12800 (2602799) 2602799..2603452 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
PIC30_RS12805 (2603445) 2603445..2604329 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
PIC30_RS12810 (2604342) 2604342..2607389 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T267789 WP_000605675.1 NZ_CP116071:2602008-2602286 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT267789 WP_000781370.1 NZ_CP116071:2602286-2602570 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References