Detailed information of TA system TA026775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 18659..19387
• Replicon: plasmid pJE01
• Accession: NC_017442
• Organism: Erwinia sp. Ejp617

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EJP617_RS17930
• Protein ID: WP_041473956.1
• Coordinates: 19073..19387 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EJP617_RS17925
• Protein ID: WP_011113999.1
• Coordinates: 18659..19072 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP617_RS17910	13889..14746	-	858	WP_011113996.1	LysR family transcriptional regulator	-
EJP617_RS17915	14887..16272	+	1386	WP_041474748.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
EJP617_RS17920	16595..18151	-	1557	WP_011113998.1	MCP four helix bundle domain-containing protein	-
EJP617_RS17925	18659..19072	-	414	WP_011113999.1	helix-turn-helix domain-containing protein	Antitoxin
EJP617_RS17930	19073..19387	-	315	WP_041473956.1	type II toxin-antitoxin system HigB family toxin	Toxin
EJP617_RS17935	19651..19935	-	285	WP_011114000.1	VF530 family protein	-
EJP617_RS17940	20095..20382	-	288	WP_011078058.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EJP617_RS17945	20372..20626	-	255	WP_011078059.1	plasmid stabilization protein	-
EJP617_RS17950	20836..21801	-	966	WP_011114001.1	ThiF family adenylyltransferase	-
EJP617_RS17955	21817..22575	-	759	WP_041474749.1	thiazole synthase	-
EJP617_RS17960	22579..22776	-	198	WP_014541855.1	sulfur carrier protein ThiS	-
EJP617_RS17965	22773..23705	-	933	WP_014541856.1	FAD-dependent oxidoreductase	-
EJP617_RS19170	24081..24272	-	192	Protein_25	Txe/YoeB family addiction module toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..30866	30866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-134)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.32 Da
• Isoelectric Point: 10.2275

>T26775 WP_041473956.1 NC_017442:c19387-19073 [Erwinia sp. Ejp617]
MHLISRSMIKDATDKYPQHSRELLALMIAIEKGYFKAPEALKAAFPSLDNLKYLDKHYVINIGRNDLRLVALVFFESQKF
YVRHVFTHKEYDVFTALHRTKGKK

Nucleotide

• Length: 315 bp

>T26775 NC_017442:c19387-19073 [Erwinia sp. Ejp617]
ATGCACTTGATAAGCCGTAGCATGATTAAGGATGCCACTGATAAGTACCCGCAGCATTCCAGGGAACTCCTGGCTCTGAT
GATCGCTATTGAAAAAGGGTACTTTAAGGCTCCCGAAGCGTTAAAGGCGGCGTTTCCATCACTGGACAATCTGAAATATT
TAGATAAGCACTACGTTATCAATATTGGCCGCAATGACTTGCGTTTAGTTGCGCTGGTATTTTTTGAGTCTCAAAAATTT
TACGTAAGACATGTGTTTACCCACAAAGAGTACGACGTATTTACAGCGTTACATCGCACAAAGGGGAAAAAATAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 14905.32 Da
• Isoelectric Point: 4.6233

>AT26775 WP_011113999.1 NC_017442:c19072-18659 [Erwinia sp. Ejp617]
MLVADIIKAGEALTAVAPFLAGITDKRQHAEALEFVEHLLLNDPENPLLDLVCAKITAYEDTAPEFAEFNAQLAAMPAGV
AMIRLLLDQHDLTLSDLPEIGSKSMVSRVLNGERKLTLEHAKKLATRFAISPALFID

Nucleotide

• Length: 414 bp

>AT26775 NC_017442:c19072-18659 [Erwinia sp. Ejp617]
ATGCTCGTAGCCGACATCATCAAAGCCGGAGAGGCTTTAACCGCCGTTGCCCCTTTCCTTGCGGGTATTACTGACAAACG
GCAACATGCAGAGGCGCTGGAATTTGTGGAGCACCTTTTACTTAACGACCCGGAAAACCCACTTTTAGATCTGGTTTGTG
CCAAAATCACGGCTTACGAAGACACAGCGCCCGAATTCGCAGAATTTAATGCACAGCTGGCAGCCATGCCGGCAGGCGTT
GCTATGATCCGTTTGCTGCTCGATCAGCACGATTTAACGTTGTCCGATTTACCCGAAATCGGTAGCAAATCGATGGTTTC
CCGCGTACTCAATGGCGAGCGTAAACTGACCCTGGAACATGCCAAAAAGTTAGCAACGCGGTTTGCCATATCTCCGGCGT
TGTTCATCGATTAA