Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 68166..68419 Replicon plasmid p812A1-69K
Accession NZ_CP116048
Organism Escherichia coli strain EC812A1

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag PIH14_RS24075 Protein ID WP_001312851.1
Coordinates 68270..68419 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 68166..68225 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PIH14_RS24050 (64893) 64893..65639 + 747 WP_000205770.1 conjugal transfer pilus acetylase TraX -
PIH14_RS24055 (65694) 65694..66254 + 561 WP_000139328.1 fertility inhibition protein FinO -
PIH14_RS24060 (66386) 66386..66586 + 201 WP_015059022.1 hypothetical protein -
PIH14_RS24065 (66972) 66972..67571 + 600 WP_032083981.1 PIN domain-containing protein -
PIH14_RS24070 (67633) 67633..67965 + 333 WP_152916585.1 hypothetical protein -
- (68166) 68166..68225 - 60 NuclAT_1 - Antitoxin
- (68166) 68166..68225 - 60 NuclAT_1 - Antitoxin
- (68166) 68166..68225 - 60 NuclAT_1 - Antitoxin
- (68166) 68166..68225 - 60 NuclAT_1 - Antitoxin
PIH14_RS24075 (68270) 68270..68419 + 150 WP_001312851.1 Hok/Gef family protein Toxin
PIH14_RS24080 (68703) 68703..68951 + 249 WP_000083837.1 replication regulatory protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtB / blaTEM-1B / blaCTX-M-55 - 1..69262 69262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T267745 WP_001312851.1 NZ_CP116048:68270-68419 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

Antitoxin


Download         Length: 60 bp

>AT267745 NZ_CP116048:c68225-68166 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References